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Many technologies can be used to solve the clean water crisis. One of the technologies is desalination, but this technology is expensive. So that, it is necessary to find cheaper desalination technology and easier to operate. Bio-desalination is a technology that utilizes bacteria to remove sodium and chloride ions in seawater. In this research, the application of the phytotechnology concept for bio-desalination reactors was carried out. Bio-desalination technology used the uniqueness of mangrove plants (Avicennia marina) in a reed bed system reactor that adapted from a reed bed system commonly used in constructed wetlands (CWs). The aim of the research was to determine the uptake of sodium and chloride ions by Avicennia marina in a reed bed system bio-desalination reactor. The namely of reactor were AM15‰, AM25‰, AMVA15‰ and AMVA25‰. The VA code was shown that Vibrio alginolyticus addition in this reactor. The artificial saline water with initial salinity of 15‰ and 25‰ was chosen based on our previous study. Parameter of salinity was determined using salinometer. Concentration of sodium and chloride ions were analyses using ion chromatography. Based on the results, the concentration of sodium and chloride ions in Avicennia marina at AM15‰ were 843.18 mg/kg and 4959.96 mg/kg, respectively. Meanwhile, those were 1410.01 mg/kg and 5292.64 mg/kg at AM25 ‰, respectively. The concentration of sodium and chloride ions in AMVA15‰ were 1003.39 mg/kg and 3186.96 mg/kg, and it were 8036.43 mg/kg and 9783.91 mg/kg at AMVA25‰. The value of Bio-concentration Factor (BCF) and Translocation Factor (TF) were greater than 1, it indicated that Avicennia marina was as an hyperaccumulator plant for sodium and chloride ions. In conclusion, the Avicennia marina can be used in reed bed system bio-desalination reactor to reduce salinity