Phenotypic Characterization and Genetic mapping of Split-hull Mutant in Rice

학위논문 (석사)-- 서울대학교 대학원 : 식물생산과학부(작물생명과학전공), 2012. 2. 고희종.A split-hull mutant was induced by treating a chemical mutagen, N-methyl-N-nitrosourea (MNU), in the fertilized egg cells of Hwaseonchal. The hull of this split-hull mutant exhibited a gap between lemma and palea during grain filling period. This study was carried out to characterize the split-hull mutant and to construct a genetic map of the gene controlling this split-hull phenotype. In the mutant, hull splitting initiated about 15 days after flowering (DAF). The percentage of split-hull grain in a mutant panicle was about 30 percent. The cause of hull-splitting in mutant was reduced hull size compared to wild type. Genetic segregation ratio of the F2 population derived from the crossing split-hull mutant with Hwaseonchal was fitted to 3:1 ratio (wild type to split-hull type). This indicated that the split-hull trait is controlled by single recessive gene. For detecting the gene controlling the split-hull trait in the mutant, the genetic map was constructed using the F2 population derived from the cross between the split-hull mutant and Milyang 23. The BSA (Bulked segregant analysis) revealed that the gene is linked to two STS markers which are located in chromosome 4.INTRODUCTION 1 _x000D_ MATERIALS AND METHODS 3_x000D_ 1. Plant materials 3 _x000D_ 2. Evaluations of split-hull trait and grain characters 3 _x000D_ 3. Statistical analysis 4 _x000D_ 4. Genetic analysis 4 _x000D_ RESULTS 7_x000D_ 1. Morphological analysis 7_x000D_ 2. Genetic analysis 14 _x000D_ DISCUSSION 21 _x000D_ LITERATURE CITED 24 _x000D_ ABSTRACT IN KOREAN 28Maste