Spatial-genetic analysis of sexual reproduction of a population of a short-toothed sage (Salvia brachyodon Vandas) from Pelješac peninsula

Kratkozupčasta kadulja (Salvia brahyodon Vandas) je endemična biljna vrsta iz porodice usnača (Lamiaceae), koja je uz spolno, karakterizirana i naglašenim nespolnim (klonalnim) razmnožavanjem. Ovaj diplomski rad proveden je u sklopu opsežnijeg istraživanja kojim će se provesti detaljna analiza spolnog razmnožavanja najveće populacije ove vrste, smještene na poluotoku Pelješcu (Dalmacija, Hrvatska). Za potrebe ovog rada, analizom osam mikrosatelitnih lokusa provedena je genotipizacija 245 uzoraka sjemenskog materijala porijeklom s 14 odabranih majčinskih biljaka. Rezultati ovog istraživanja ukazuju na prisutnost značajne razine samooplodnje unutar istraživane populacije, dok istovremeno negativan utjecaj samooplodnje na ukupnu genetsku varijabilnost populacije nije zabilježen. Također, analizirani set sjemenki je karakteriziran razmjerno visokim razinama očekivane i uočene heterozigotnosti te alelnog bogatstva, što je u skladu s vrijednostima na razini čitave populacije dobivenima u ranijim istraživanjima. Povezanost između specifične prostorno-genetičke strukture populacije i njezinog spolnog razmnožavanja tj. zabilježenih razina samooplodnje nije utvrđena, vjerojatno zbog premalog broja analiziranih jedinki. Na posljetku, utvrđeni prosječni doseg polinatora, tj. udaljenost između donora i akceptora polena iznosi 32,3 m iz čega proizlazi zaključak da doseg oprašivača ne može predstavljati ograničavajući faktor za stranooplodnju unutar istraživane populacije.The short-toothed sage (Salvia brahyodon Vandas) is an endemic plant species from the Lamiaceae family. In addition to sexual intercourse, it is characterized and recognized by asexual (clonal) reproduction. This thesis includes a framework of extensive research that investigates and produces a detailed analysis of the plants sexual reproduction as the largest population of this species, which is located on the Pelješac peninsula (Dalmatia, Croatia). For the purpose of this work, the analysis of eight microsatellite loci was performed by genotyping 245 samples of seed material originating from 14 selected mother plants. The results of this study indicate the presence of significant levels of self-fertilization within the studied populations, while active negative self-fertilization on the total genetic variability of the population was not recorded. Also, the analyzed set of seeds was characterized by relatively high various expectations, heterozygosity and allelic richness, which is consistent with the value at the level of read populations obtained in previous studies. The association between specific spatial-genetic structures of the population and its sexual reproduction, in other words the recorded level of self-fertilization, has not been established. This is probably due to the small number of analyzed individuals. Finally, the average doses of pollinators were determined. The distance between the donor and the pollen acceptor is 32.3 m, from which it was concluded that the dose of pollinators cannot be a limiting factor for in vitro fertilization within the studied populations