To make the EGFRvIII mutant cell line in H9, gene editing was performed using CRISRP-CAS9 with two sgRNAs. This is the sequencing data for EGFR site for mutant cell line. Off-target position of each sgRNA and deep sequencing raw dat
The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (g...
<p>Sequence of sgRNAs and primers used for CRISPR/Cas9 knockout cell generation and evaluation.</p
(a) Schematic diagram of the TGFBI partial protein coding region and the locus targeted by the sgRNA...
To make the EGFRvIII mutant cell line in H9, gene editing was performed using CRISRP-CAS9 with two s...
<p><b>A</b>. Schematic diagram of components between the start and second codons of <i>EGFP</i> cDNA...
<p>Unsynchronized or synchronized and released cells were transfected via electroporation with CRISP...
We developed a method to detect copy number variation and loss of heterozygosity in genome-edited ce...
Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to...
<p><b>(A) Allelic analysis of eGFP positive cells expanded as a clonal population.</b> Clonally isol...
<p>A. Schematic of sequencing primers and areas of interest. Arrows indicate the location of the pri...
The data was obtained in course of performing a CRISPR/Cas9 knock-out in human polarized epithelial ...
<p>Five cloned DNA fragments of 250 bp containing DNA from PCR amplified sgRNA target regions of (pr...
CRISPR/Cas9 has demonstrated a high-efficiency in site-specific gene targeting. However, potential o...
<p>Gene editing in human embryonic stem cells (hESCs) has been significantly enhanced by the discove...
Additional file 1: Figure S1. A schematic illustration for constructing a Cas9, eGFP and gRNA co-exp...
The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (g...
<p>Sequence of sgRNAs and primers used for CRISPR/Cas9 knockout cell generation and evaluation.</p
(a) Schematic diagram of the TGFBI partial protein coding region and the locus targeted by the sgRNA...
To make the EGFRvIII mutant cell line in H9, gene editing was performed using CRISRP-CAS9 with two s...
<p><b>A</b>. Schematic diagram of components between the start and second codons of <i>EGFP</i> cDNA...
<p>Unsynchronized or synchronized and released cells were transfected via electroporation with CRISP...
We developed a method to detect copy number variation and loss of heterozygosity in genome-edited ce...
Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to...
<p><b>(A) Allelic analysis of eGFP positive cells expanded as a clonal population.</b> Clonally isol...
<p>A. Schematic of sequencing primers and areas of interest. Arrows indicate the location of the pri...
The data was obtained in course of performing a CRISPR/Cas9 knock-out in human polarized epithelial ...
<p>Five cloned DNA fragments of 250 bp containing DNA from PCR amplified sgRNA target regions of (pr...
CRISPR/Cas9 has demonstrated a high-efficiency in site-specific gene targeting. However, potential o...
<p>Gene editing in human embryonic stem cells (hESCs) has been significantly enhanced by the discove...
Additional file 1: Figure S1. A schematic illustration for constructing a Cas9, eGFP and gRNA co-exp...
The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (g...
<p>Sequence of sgRNAs and primers used for CRISPR/Cas9 knockout cell generation and evaluation.</p
(a) Schematic diagram of the TGFBI partial protein coding region and the locus targeted by the sgRNA...
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