Antifungal activity of essential oils against fungus Pyrenophora graminea

Cilj ovoga istraživanja je ispitati utjecaj dvanaest eteričnih ulja na porast micelija gljive Pyrenophora graminea . Eterična ulja su primjenjivana u količinama od 5, 10, 15, 25 i 50µl. Pokus je postavljen u tri ponavljanja. Eterična ulja primijenjen u količini 25 i 50µl su statistički vrlo značajno inhibirala porast micelija gljive u odnosu na kontrolu. Mjerenje zone inhibicije provedeno je četvrtog i sedmog dana od inokulacije. Najbolji antifungalni učinak imalo je ulje timijana. Ulje timijana je potpuno inhibiralo porast micelija gljive Pyrenophora graminea. Pored ulja timijana, eterična ulja citronele, lavande, čajevca, anisa i cimet kore su imala jak antifungalni učinak. Eterično ulje eukaliptusa ima najslabiji antifungalni učinak.The aim of this study was to determine the effect of twelve essential oils on the mycelial growth of Pyrenophora graminea. Essential oils are applied in amounts of 5, 10, 15, 25 and 50µl. The experiment was performed in the three repetitions. The inhibition zone was measured fourth and seventh days after inoculation. Essential oils applied in the amount of 25 and 50µl statistically very significantly inhibited the growth of fungal mycelium compared to the control. Thyme oil had the best antifungal effect. Thyme oil completely inhibited the growth of mycelium of the fungus Pyrenophora graminea. In addition to thyme oil, the essential oils of citronella, lavender, tea tree, anise and cinnamon bark had a strong antifungal effect. Eucalyptus essential oil had the weakest antifungal activity