Determination of lutein in eggs enriched with functional ingredients

Sadržaj luteina određivan je u ukupno šest skupina jaja. Četiri skupine nesilica hranjene su krmnim smjesama različitog sastava od kojih je u krmnu smjesu kojom su hranjene nesilice za jednu skupinu kao funkcionalni sastojak dodan samo lutein, dok je u krmnu smjesu za drugu skupinu nesilica, uz druge funkcionalne sastojke dodan i lutein. Dvije skupine jaja proizvedena su na dva obiteljska poljoprivredna gospodarstva. Lutein je određivan u žumanjku jaja korištenjem tekućinske kromatografije visoke razlučivosti s UV detekcijom. Najmanja koncentracija luteina određena je u kontrolnoj skupini i iznosila je 0,644 mg/100 g žumanjka dok je najveća koncentracija luteina 3,661 odnosno 3,789 mg/100 g žumanjka, određena u skupinama jaja koja su dobivena od nesilica koje su hranjene krmnim smjesama u koje je dodan lutein. Usporedbom s literaturnim podacima, u ove dvije skupine koncentracija luteina je povećana za oko šest puta. U jajima koja su proizvedena na dva obiteljska poljoprivredna gospodarstva, koncentracija luteina je povećana za šest odnosno četiri puta.Lutein content was determined in a total of six groups of eggs. Four groups of laying hens were fed with feed mixtures of different composition, lutein was the only added functional ingredient to the feed mixture for one group of laying hens, while lutein with other functional ingredients was added to the feed mixture for another group of laying hens. Two groups of eggs were produced on two family farms. Lutein was determined in egg yolks using high-performance liquid chromatography with UV detection. The lowest concentration of lutein was determined in the control group and was 0.644 mg / 100 g of yolk, while the highest concentration of lutein was 3.661 and 3.799 mg / 100 g of yolk, determined in groups of eggs obtained from laying hens fed with feed mixtures enriched with lutein. Compared with the literature data in these two groups, the lutein concentration was increased by about six times. In eggs produced on two family farms, the lutein concentration was increased by six and four times, respectively