Activity of esterase in Pseudomonas sp. cells without and with induction by erythromycin

U ovome radu praćena je aktivnost enzima eritromicin esteraze prilikom uzgoja bakterije Pseudomonas sp. Eritromicin esteraza je enzim koji navedena bakterija sintetizira, a koji razgrađuje eritromicin; antibiotik široke primjene. Aktivnost enzima je praćena tijekom uzgoja bakterije bez dodatka eritromicina u hranjivu podlogu, s dodatkom 0,2 ppm te 200 ppm eritromicina u hranjivu podlogu. Cilj rada bio je izolirati enzim iz stanica Pseudomonas sp. uzgojenih bez, s dodatkom 0,2 ppm i dodatkom 200 ppm eritromicina u hranjivu podlogu te provesti reakciju razgradnje eritromicina.Activity of erythromycin esterase was monitored during cultivation of Pseudomonas sp. Erythromycin esterase is an enzyme which degrades erythromycin, widely used antibiotic. Enzyme activity was monitored during microbial cultivation in the system without added erythromycin in culture medium, with added 0.2 ppm and 200 ppm of erythromycin. The aim of the work was to isolate the enzyme from Pseudomonas sp. cells cultivated without, with adding 0.2 ppm and 200 ppm of erythromycin in culture medium and to carry out the erythromycin degradation reaction with the same enzyme