Determination of lidocaine degradation products by high resolution mass spectrometry

U ovom diplomskom radu razvijena je učinkovita metoda tekućinske kromatografije ultra visoke djelotvornosti (UHPLC) za analizu onečišćenja i razgradnih produkata lidokaina. Razvoj metode uključivao je testiranje različitih nepokretnih faza, modifikatora pokretnih faza i različite omjere otapala u pokretnoj fazi. Razvijenom metodom uspješno su odijeljeni pikovi lidokaina, onečišćenja i razgradnih produkata. Na temelju nastalih fragmenata pretpostavljene su strukture razgradnih produkata. Određeni su i mogući načini fragmentacije razgradnih produkata lidokaina na temelju MS/MS spektara.A quick and efficient method of high efficiency liquid chromatography (UHPLC) for analysis of contaminants and degradation products of the drug lidocaine was developed through this diploma thesis. The development of the method involved the testing of various fixed phases, mobile phase modifiers and different proportions of the solvent in the mobile phase. The developed methods had successfully separated peaks of lidocaine from contaminants and degradation products. Structure of the degradation products were assumed based on the resulting fragments. There were also assessed possible modes of lidocaine degradation products fragmentation by analyzing MS/MS spectra