The azido[\u3csup\u3e14\u3c/sup\u3eC]atrazine photoaffinity technique labels a 34-kDa protein in Scenedesmus which functions on the oxidizing side of photosystem II

We have used azido[14C]atrazine to photoaffinity label thylakoids from wild-type (WT) Scenedesmus and a mutant, LF-1, which is blocked on the oxidizing side of photosystem II (PS II). One protein is labeled in each case, at 34 kDa in the WT and 36 kDa in LF-1. Previous comparison of the WT with LF-1 had been used to assign a PS II donor side function to the 34-kDa protein. These results suggest that this photoaffinity technique does not label the herbicide-binding protein involved in electron transfer on the reducing side of PS II. © 1985