Sonothrombolysis with magnetically targeted microbubbles

This data underpins the following paper: de Saint Victor et al., Sonothrombolysis with magnetically targeted microbubbles, Ultrasound in Medicine & Biology, 2019. There are 5 sections: 1) Magnetic field caracterisation: magnitude of the magnetic field and of the magnetic field gradient in the experimental setup (used in figure 2) 2) Accumulation of magnetic material in the focal region with and without a magnet (used in figure 3) 3) Lysis rates (used in figures 4 and 8) 4) Acoustic energy detected (used in figures 7 and 8) 5) Clot debris sizing Microbubble-enhanced sonothrombolysis is a promising approach to increase the safety and efficacy of current pharmacological treatments for ischemic stroke. Maintaining therapeutic concentrations of microbubbles and drugs at the clot site however poses a challenge. The objective of this study was to investigate the efficacy of magnetic microbubble targeting upon clot lysis rates in vitro. Retracted whole porcine blood clots were placed in a flow phantom of a partially occluded middle cerebral artery. The clots were treated with a combination of tissue plasminogen activator (0.75µg/mL), magnetic microbubbles (~107 microbubbles/mL), and ultrasound (0.5MHz, 630kPa peak rarefactional pressure, 0.2Hz pulse repetition frequency, 2% duty cycle). Magnetic targeting was achieved using a single permanent magnet element (0.08-0.38T and 12-140T/m in the region of the clot). The change in clot diameter was measured optically over the course of the experiment. Magnetic targeting produced a three-fold average increase in lysis rates and linear correlation was observed between lysis rate and total energy of acoustic emissions