Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries

  • Matysiak, S
  • Hellmuth, K
  • El-Sagheer, A
  • Shivalingam, A
  • Ariyurek, Y
  • de Jong, M
  • Hollestelle, M
  • Out, R
  • Brown, T
Publication date
January 2017
Publisher
Royal Society of Chemistry (RSC)

Abstract

DNA encoded ligands are self-assembled into bivalent complexes and chemically ligated to link their identities. To demonstrate their potential as a combinatorial screening platform for avidity interactions, the optimal bivalent aptamer design (examplar ligands) for human alpha-thrombin is determined in a single round of selection and the DNA scaffold replaced with minimal impact on the final design

Extracted data

We use cookies to provide a better user experience.