The application of a simple tissue-disruption method to DNA extraction for species identification

The TissueLyser II was used to disrupt animal bones and plant tissue without dry ice or liquid nitrogen for species identification. Bone fragments of fresh swine (Sus scrofa) ribs were used. A 521bp fragment of the cyt b locus was analyzed, and the BLASTn results confirmed that the origin of all the samples was swine (E-value = 0.0). Bone fragments of bovine, chicken, duck, fish, sheep, and swine were also used to test the procedure. An approximately 600bp fragment of the COI locus was analyzed, and the BLASTn results confirmed the origin of all the samples (E-value = 0.0). Fresh plant samples from NYC and known plant samples with various conditions were used. An approximately 600bp fragment of the rbcL locus was analyzed, and a combination of BLASTn, botanical database (NYBG and NYFA) searches, and morphological analysis were used to determine the species of the samples. Fresh plant samples were successfully sequenced, while known plant samples with various conditions yielded poor quality sequencing results