Add to ORKGCurrent gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes
The predictability of DNA self-assembly is exploited in many nanotechnological approaches. Inspired ...
We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to l...
Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures form...
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthes...
Biochemical strategies that use a combination of synthetic oligonucleotides, thermostable DNA polyme...
The chemical synthesis of oligonucleotides and their enzyme-mediated assembly into genes and genomes...
DNA assembly techniques have developed rapidly, enabling efficient construction of complex construct...
DNA assembly techniques have developed rapidly, enabling efficient construction of complex construct...
In principle, a pre-constructed library of all possible short oligonucleotides could be used to cons...
An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of...
An isothermal, single-reaction protocol for assembling multiple, overlapping DNA molecules by the co...
Molecular machines that assemble polymers in a programmed sequence are fundamental to life. They are...
The programmed self-assembly of nanostructures from well-defined units is an important aim in nanosc...
We demonstrate an approach that allows attachment of single-stranded DNA (ssDNA) to a defined residu...
The molecular properties of the phosphodiester backbone that made it the evolutionary choice for the...
The predictability of DNA self-assembly is exploited in many nanotechnological approaches. Inspired ...
We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to l...
Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures form...
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthes...
Biochemical strategies that use a combination of synthetic oligonucleotides, thermostable DNA polyme...
The chemical synthesis of oligonucleotides and their enzyme-mediated assembly into genes and genomes...
DNA assembly techniques have developed rapidly, enabling efficient construction of complex construct...
DNA assembly techniques have developed rapidly, enabling efficient construction of complex construct...
In principle, a pre-constructed library of all possible short oligonucleotides could be used to cons...
An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of...
An isothermal, single-reaction protocol for assembling multiple, overlapping DNA molecules by the co...
Molecular machines that assemble polymers in a programmed sequence are fundamental to life. They are...
The programmed self-assembly of nanostructures from well-defined units is an important aim in nanosc...
We demonstrate an approach that allows attachment of single-stranded DNA (ssDNA) to a defined residu...
The molecular properties of the phosphodiester backbone that made it the evolutionary choice for the...
The predictability of DNA self-assembly is exploited in many nanotechnological approaches. Inspired ...
We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to l...
Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures form...