AldoHcy Hydrolase of Trichomonas vaginalis: Studies of the effects of 5\u27-deoxy-5\u27-(iodomethylene) adenosine and related 6-N-cyclopropyladenosine analogues on in vitro culture

Trypanosoma brucei and Trichomonas vaginalis are both parasitic protozoans that are known to share many similar biochemical pathways. Aristeromycin, as well as 5′-iodovinyl and 5′-oxime analogues of adenosine, are potent inhibitors of AdoHcy hydrolase in T. brucei, an enzyme that catalyses the hydrolysis of AdoHcy to adenosine and l-homocysteine. To help determine the role of this enzyme in T. vaginalis, we have tested a library of 5′-modified adenosine derivatives, including 5′-deoxy-5′-(iodomethylene)-adenosine and related 6-N-cyclopropyl analogues. Our results indicate that these inhibitors are effective at inhibiting the growth of T. vaginalis, by as much as 95