Chromatographic Identification of Big Sagebrush Seed

Paper and thin-layer chromatography of big sagebrush seed provides a rapid, simple means of identifying more palatable forms of this shrub. Methanol extraction of seed for 24 hr followed by two-dimensional paper (n-butanol:acetone:water, 4:1:3; acetic acid: water; 15:85) or single-dimensional thin-layer chromatography (chloroform:methanol:water; 85:10:5) reveals distinctive differences between Artemisia tridentata subsp. tridentata and the more palatable subsp. vaseyana and wyomingensis. A bright, iridescent blue spot characterizes the more palatable subspecies; the same spot is much smaller and duller in A. tridentata subsp. tridentata.This material was digitized as part of a cooperative project between the Society for Range Management and the University of Arizona Libraries.The Journal of Range Management archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact lbry-journals@email.arizona.edu for further information.Migrated from OJS platform August 202