Small RNAs are widespread in plants and animals. They largely include microRNAs (miRNAs) and short interfering RNAs (siRNAs), and they play key roles in gene and chromatin regulations. Here we describe in detail the method for an effective construction of the recently developed short tandem target mimic (STTM) technology to block small RNA functions in plants and animals. STTM is a powerful technology complementing the previous target mimic (TM) in plants and the miRNA sponge, as well as the recently defined endogenous competing RNA (CeRNA) in animals. We expect STTM will not only be effective in blocking small RNA functions in plants but will also become a popular approach in animals. © 2012 Elsevier Inc
Many targets of plant microRNAs (miRNAs) are thought to play important roles in plant physiology and...
Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syntasiRNAs) are t...
The challenge of understanding the widespread biological roles of animal microRNAs (miRNAs) has prom...
MicroRNAs (miRNAs) and other endogenous small RNAs act as sequence-specific regulators of the genome...
© Springer Science+Business Media LLC 2017. Small RNAs, including microRNAs (miRNAs), are abundant i...
microRNAs (miRNAs) are endogenous small non-coding RNAs that bind to mRNAs and target them for cleav...
In plants, microRNAs (miRNAs) regulate more than hundred target genes comprising largely transcripti...
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in gene regulation. T...
microRNAs are post-transcriptional regulators of gene expression that recruit RNA silencing com-plex...
Small non-coding RNA includes miRNAs (microRNAs) and siRNAs (interfering RNAs), which act as regulat...
In plants, double-stranded RNA (dsRNA) is an effective trigger of RNA silencing, and several classes...
In plant cells, DICER-LIKE4 processes perfectly double-stranded RNA (dsRNA) into short interfering (...
MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-tra...
The process of RNA interference allows cells to regulate functions of their genes. This process is u...
Artificial small RNAs (sRNAs) are short ?21-nt non-coding RNAs engineered to inactivate seque...
Many targets of plant microRNAs (miRNAs) are thought to play important roles in plant physiology and...
Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syntasiRNAs) are t...
The challenge of understanding the widespread biological roles of animal microRNAs (miRNAs) has prom...
MicroRNAs (miRNAs) and other endogenous small RNAs act as sequence-specific regulators of the genome...
© Springer Science+Business Media LLC 2017. Small RNAs, including microRNAs (miRNAs), are abundant i...
microRNAs (miRNAs) are endogenous small non-coding RNAs that bind to mRNAs and target them for cleav...
In plants, microRNAs (miRNAs) regulate more than hundred target genes comprising largely transcripti...
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in gene regulation. T...
microRNAs are post-transcriptional regulators of gene expression that recruit RNA silencing com-plex...
Small non-coding RNA includes miRNAs (microRNAs) and siRNAs (interfering RNAs), which act as regulat...
In plants, double-stranded RNA (dsRNA) is an effective trigger of RNA silencing, and several classes...
In plant cells, DICER-LIKE4 processes perfectly double-stranded RNA (dsRNA) into short interfering (...
MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-tra...
The process of RNA interference allows cells to regulate functions of their genes. This process is u...
Artificial small RNAs (sRNAs) are short ?21-nt non-coding RNAs engineered to inactivate seque...
Many targets of plant microRNAs (miRNAs) are thought to play important roles in plant physiology and...
Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syntasiRNAs) are t...
The challenge of understanding the widespread biological roles of animal microRNAs (miRNAs) has prom...