Purification of mouse interferon by sequential chromatography

Two schemes for the purification of mouse interferon are described, based on the concerted application of various physicochemical and affinity/adsorption column chromatographic techniques. Mouse interferon was purified to a final specific activity of 1.0--8.0 x 10(8) units/mg when first precipitated with ammonium sulphate and further processed by hydrophobic chromatography and adsorption chromatography on AFFI-Gel 202 and Controlled Pore Glass. It was purified to a final specific activity of 2.5--3.7 x 10(8) units/mg when first precipitated with ammonium sulphate and further processed by gel filtration with Ultrogel AcA 54, ion-exchange chromatography with Carboxymethyl Boi-Gel Agarose, hydrophobic chromatography with AFFI-Gel 202 and adsorption chromatography with Controlled Pore Glass.status: publishe