An assessment of histone-modification antibody quality
- Egelhofer, Thea A
- Minoda, Aki
- Klugman, Sarit
- Lee, Kyungjoon
- Kolasinska-Zwierz, Paulina
- Alekseyenko, Artyom A
- Cheung, Ming-Sin
- Day, Daniel S
- Gadel, Sarah
- Gorchakov, Andrey A
- Gu, Tingting
- Kharchenko, Peter V
- Kuan, Samantha
- Latorre, Isabel
- Linder-Basso, Daniela
- Luu, Ying
- Ngo, Queminh
- Perry, Marc
- Rechtsteiner, Andreas
- Riddle, Nicole C
- Schwartz, Yuri B
- Shanower, Gregory A
- Vielle, Anne
- Ahringer, Julie
- Elgin, Sarah C R
- Kuroda, Mitzi I
- Pirrotta, Vincenzo
- Ren, Bing
- Strome, Susan
- Park, Peter J
- Karpen, Gary H
- Hawkins, R David
Publication date
January 2011
DOIAbstract
We report testing of the specificity and utility of over 200 antibodies raised against 57 different histone modifications, in Drosophila melanogaster, Caenorhabditis elegans and human cells. While most antibodies performed well, over 25% failed specificity tests by dot blot or western blot. Among specific antibodies, over 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use and provide a website for posting new test results
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