Cleavage of histone pre-mRNAs at the 3′ end is guided by the U7 snRNP, which is a component of a larger 3′-end processing complex. To identify other components of this complex, we isolated proteins that stably associate with a fragment of histone pre-mRNA containing all necessary processing elements and a biotin affinity tag at the 5′ end. Among the isolated proteins, we identified three well-characterized processing factors: the stem-loop binding protein (SLBP), which interacts with the stem-loop structure upstream of the cleavage site, and both Lsm11 and SmB, which are components of the U7-specifc Sm ring. We also identified 3′hExo/Eri-1, a multifunctional 3′ exonuclease that is known to trim the 3′ end of 5.8S rRNA. 3′hExo primarily bind...
During 3' end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by...
During 3' end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by...
Processing of histone pre-mRNA requires a single 3′ endonucleolytic cleavage guided by the U7 snRNP ...
Cleavage of histone pre-mRNAs at the 3′ end is guided by the U7 snRNP, which is a component of a lar...
The 3′ end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after ...
The 3′ end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after ...
Metazoan replication-dependent histone pre-mRNAs undergo a unique 3′-cleavage reaction which does no...
Nearly all eukaryotic mRNAs end with a poly (A) tail that is added to their 3’ end by the ubiquitous...
Nearly all eukaryotic mRNAs end with a poly (A) tail that is added to their 3’ end by the ubiquitous...
Cleavage of histone pre-mRNAs at the 3' end requires stem-loop binding protein (SLBP) and U7 snRNP t...
SummaryDuring 3′ end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem ...
Animal replication-dependent histone pre-mRNAs are processed at the 3′ end by endonucleolytic cleava...
Animal replication-dependent histone pre-mRNAs are processed at the 3′ end by endonucleolytic cleava...
Metazoan replication dependent histone mRNAs form their 3' end by endonucleolytic cleavage of the pr...
Metazoan replication dependent histone mRNAs form their 3' end by endonucleolytic cleavage of the pr...
During 3' end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by...
During 3' end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by...
Processing of histone pre-mRNA requires a single 3′ endonucleolytic cleavage guided by the U7 snRNP ...
Cleavage of histone pre-mRNAs at the 3′ end is guided by the U7 snRNP, which is a component of a lar...
The 3′ end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after ...
The 3′ end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after ...
Metazoan replication-dependent histone pre-mRNAs undergo a unique 3′-cleavage reaction which does no...
Nearly all eukaryotic mRNAs end with a poly (A) tail that is added to their 3’ end by the ubiquitous...
Nearly all eukaryotic mRNAs end with a poly (A) tail that is added to their 3’ end by the ubiquitous...
Cleavage of histone pre-mRNAs at the 3' end requires stem-loop binding protein (SLBP) and U7 snRNP t...
SummaryDuring 3′ end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem ...
Animal replication-dependent histone pre-mRNAs are processed at the 3′ end by endonucleolytic cleava...
Animal replication-dependent histone pre-mRNAs are processed at the 3′ end by endonucleolytic cleava...
Metazoan replication dependent histone mRNAs form their 3' end by endonucleolytic cleavage of the pr...
Metazoan replication dependent histone mRNAs form their 3' end by endonucleolytic cleavage of the pr...
During 3' end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by...
During 3' end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by...
Processing of histone pre-mRNA requires a single 3′ endonucleolytic cleavage guided by the U7 snRNP ...