Quantitative PCR for Detection and Enumeration of Genetic Markers of Bovine Fecal Pollution
- Shanks, O. C.
- Atikovic, E.
- Blackwood, A. D.
- Lu, J.
- Noble, R. T.
- Domingo, J. S.
- Seifring, S.
- Sivaganesan, M.
- Haugland, R. A.
DOIAbstract
Accurate assessment of health risks associated with bovine (cattle) fecal pollution requires a reliable host-specific genetic marker and a rapid quantification method. We report the development of quantitative PCR assays for the detection of two recently described bovine feces-specific genetic markers and a method for the enumeration of these markers using a Markov chain Monte Carlo approach. Both assays exhibited a range of quantification from 25 to 2 × 106 copies of target DNA, with a coefficient of variation of <2.1%. One of these assays can be multiplexed with an internal amplification control to simultaneously detect the bovine-specific genetic target and presence of amplification inhibitors. The assays detected only cattle fecal speci...
Add to ORKG