Time-kill Effect of Polymyxin in Combination with Meropenem against Multi-Drug Resistant Pseudomonas aeruginosa

Polymyxin B based combinations may improve clinical outcomes by utilizing its rapid killing potential in conjunction with the sustained action of meropenem. The objective of the study is to evaluate the pharmacodynamic activity of polymyxin B in combination with meropenem against Pseudomonas aeruginosa clinical isolates. Antibiotics used in combination should have better pharmacodynamic killing activity against P. aeruginosa clinical isolates and should result in suppressed regrowth patterns as compared to monotherapy. In-vitro static time-kill experiments were performed using P. aeruginosa clinical isolates with differing susceptibilities, polymyxin B resistant (PBr) strain PSA215 and polymyxin B sensitive (PBs) strain PSA218 to evaluate the killing activity of polymyxin based combinations with meropenem.Doctor of Pharmac