A study of chromatographic methods

The experiments for this study were conducted in order to investigate the possibilities of satisfactorily separating mixtures of certain organic compounds by paper and thin-layer chromatographic methods. Parts I, II and III were concerned with the separation of a mixture of amino acids. In Part I the mixture of amino acids was separated on narrow chromatographic paper strips, using the ascending method. Large sheets of chromatographic paper were used in Part II for the one-dimensional and the two-dimensional descending chromatographic methods. In the one-dimensional descending method the mixture was separated by the use of a single solvent system, whereas in the two-dimensional descending method two distinct solvent systems were employed, the second running at right angles to the first. The circular ascending chromatographic method used in Part III required the use of chromatographic paper rolled into cylinders