Application of fluxomic methods to study tumor cell metabolism

Aim of the study Analysis of metabolic and transcriptional differences in healthy and tumor cells and search of antitumor targets using fluxomic methods. Objectives of the study 1. To develop the primary breast cancer cell culture. 2. To identify a therapeutic window by performing analysis of transcriptional differences between healthy and tumor cells. 3. To experimentally evaluate the therapeutic window by the inhibition of branched-chain amino acid transaminases 2 in healthy and tumor cells. 4. To determine the influence of the interaction of fatty acid synthesis and oxidation on the viability of tumor cells using 13C-labeled glutamine. Novelty of the study Cancer cells are characterized by the Warburg effect. The Warburg effect consists in the lactic fermentation of glucose even at high oxygen concentrations. This effect was initially believed to be due to defects in cancer cell mitochondria; however, this hypothesis has proven to be wrong, as mito-chondria have been shown to be functional and cancer cells cannot survive in fully anaerobic conditions, which proves that they are still dependent on oxidative phosphorylation. Recent studies have shown that the mitochondria of cancer cells function well and both oxidative phosphorylation and lactic fermentation are important for energy production