Non-enzymatic lab-on-paper devices for biosensing applications

Lab-on-paper devices constitute a promising tool for biosensing systems due to their easiness of fabrication and use, inherent low-cost, easy tailoring in terms of device architecture and/or function, biocompatibility and easy disposal. When combined with non-enzymatic approaches the increase on device's shelf life, stability and analytical features improvement can be achieved. In this chapter, a brief description of the lab-on-paper concept is presented alongside a review of works reporting non-enzymatic approaches on paper-based platforms using two of the most common transduction mechanisms: optical and electrochemical methods.The authors acknowledge funding from project PTDC/AAG-TEC/5400/2014, POCI01-0145-FEDER-016637, POCI-01-0145-FEDER-007688, UID/CTM/50025/2019 and DIGISMART ERC AdG 787410 funded by European funds, through FEDER (European funding and Regional Development) via COMPETE2020—POCI (operational program for internationalization and competitively), by national funding through the National Foundation for Science and Technology, I.P. (FCT-MCTES). ACM and ARC gratefully acknowledges FCT-MCTES for the financial support (PhD grant reference SFRH/BD/115173/2016 and SFRH/BD/130107/2017, respectively).