Understanding the individual role of fish, oyster, phytoplankton and macroalgae in the ecology of integrated production in earthen ponds

In order to demonstrate that IMTA can be profitable and a good alternative to regular semi-intensive fish mariculture production in earthen ponds three different production treatments with distinct combinations of trophic levels were designed: (i) a combination of fish, filter feeders, phytoplankton and macroalgae, (ii) fish, filter feeders and phytoplankton and (iii) fish, phytoplankton and macroalgae, to evaluate the role of each trophic level within an Integrated Multi-Trophic Aquaculture system (IMTA). Each treatment was carried out under semi-intensive conditions with two replicates, in a total of 6 earthen ponds of 500 m(2) surface and depth of 1.5 m. The results showed that fish, oyster, phytoplankton and macroalgae integrated aquaculture is a healthy sustainable production system for mariculture in earthen ponds, providing much more fish supply compared with the other two treatments. Ponds with filter feeders had significantly lower turbidity (Nephelometric Formazin unit (FNU) of 13 in the morning and 17 in the afternoon) when compared to ponds without filter feeders (16 FNU in the morning and 20 FNU in the afternoon) with increased light penetration throughout water column (61 and 55 cm transparency in ponds with filter feeders compared to 51 cm in ponds without filter feeders) and consequently higher photosynthetic activity with significantly higher dissolved oxygen (5.4 mg L in the morning and 6.7 mg L-1 in the afternoon in ponds with filter feeders compared to 5.3 mg L-1 in the morning and 6.4 mg L-1 in the afternoon in ponds without filter feeders) and carbon sequestration (0.50 and 0.53 mg L-1 8 h(-1) in ponds with filter feeders and 0.43 mg L-1 8 h(-1) in ponds without filter feeders). In the fish, filter feeder, phytoplanton and macroalgae IMTA treatment, phytoplankton played a crucial role because they increased DO levels, removed the excess of nutrients from animal excretion, and was used as food by the filter feeders. Almost as important is the presence of filter feeders since they control the density of the microalgae and particulate matter in the ponds contributing to a more constant level of DO and higher transparency of the water column. The increased transparency and pond fertilization by oyster excretion, resulted in higher proliferation of phytoplankton (chlorophyll a concentrations of 16.5 mu gL(-1) and 20.2 mu g L-1 in ponds with filter feeder and 13.3 mu g L-1 in ponds without filter feeder) with benefits not only for filter feeders themselves but also for the macroalgae. At the end there was higher water quality and higher savings (14% day(-1)) in the energy costs for pond aeration. Meagre, white seabream and flathead grey mullet enhance their performance in IMTA systems with the presence of filter feeders with food conversion rates (FCR) of 1.52 when compared with 2.46 in the regular semi-intensive system composed by fish, phytoplankton and macroalgae. Meagre grew significantly more in IMTA systems with controlled macroalgae while white seabream and flathead grey mullet enhance their performance in the presence of macroalgae. The results show that the fish, oyster, phytoplankton and macroalgae integrated production in earthen ponds is an improved system compared to the regular semi-intensive fish production. The enhanced water quality in these systems leads to improved fish performance and higher biomass production, and to reduction in the energy power used, contributing to greater profitability.Fundacao para a Ciencia e TecnologiaPortuguese Foundation for Science and Technology [COFASP/0003/2015]project DIVERSIAQUA [Mar2020 16-02-01-FMP-0066]project Algared+ [Poctep 0055_ALGARED_PLUS_5_E]Fundacao para a Ciencia e Tecnologia, PortugalPortuguese Foundation for Science and Technology [SFRH/BD/118601/2016][UID/Multi/04326/2019