Biology of teleost primordial germ cells (PGCs) and spermatogonia: Biotechnological applications

This review provides a general view on teleost germline development using primordial germ cells (PGCs) or spermatogonia (SG), highlighting recent progress in research on these two cell types in teleost fishes. Due to the interest of these cells for gene banking purposes, this chapter also reviews the available protocols for cryopreservation and the techniques that have been employed to assess cellular and molecular status after the process. Protocols for in vitro culture and the possibility of generating PGCs in vitro from non-committed embryonic cells are also discussed. Furthermore, the potential of these cells in surrogate production is presented, analyzing the transplantation and xenotransplantation experiments performed in fish. (C) 2016 Elsevier B.V. All rights reserved.COST Office (Food and Agriculture COST Action) [FA1205: AQUAGAMETE]Fundacion Ramon ArecesMinisterio de Ciencia e Innovacion [MICINN AGL2009-06994, AGL2015-68330]Ramon y Cajal Program [RYC-2008-02339]Junta de Castilla y Leon [E-24-2009-0036681 EDU/1084/2012]Ministry of Education, Youth and Sports of the Czech Republic [CZ.1.05/2.1.00/01.0024, LO1205]Czech Science Foundation [P502/13/26952S]