Improvement of enzymatic performance of Asclepias curassavica L. proteases by immobilization: Application to the synthesis of an antihypertensive peptide
- Origone, Anabella Lucía
- Barberis, Sonia Esther
- Illanes, Andrés
- Guzmán, Fanny
- Camí, Gerardo Enrique
- Liggieri, Constanza Silvina
- Martínez, Ronny
- Bernal, Cintia Claudia
DOIAbstract
The aim of this work was to study different immobilization strategies on silica supports in order to obtain robust biocatalysts from latex proteases of Asclepias curassavica L., a South American native plant. Immobilized enzyme performance was evaluated under harsh reaction conditions such as the synthesis of the antihypertensive peptide N-α-CBZ-Val-Gly-OH. Proteases from A. curassavica, named asclepain, were immobilized (0.51–5.56 mg of protein/ g of support) in non-functionalized silica (S), in glyoxyl-silica (GS) and in octyl-glyoxyl-silica (OGS), by adsorption, and multi-point covalent attachment on mono and hetero-functional supports, respectively, under previously determined optimal immobilization conditions. Immobilization yields wer...
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