17β-Estradiol stimulates the translocation of endogenous estrogen receptor α at the plasma membrane of normal anterior pituitary cells
- Gutiérrez, Silvina
- Sosa, Liliana del Valle
- Petiti, Juan Pablo
- Mukdsi, Jorge Humberto
- Mascanfroni, Ivan Darío
- Pellizas, Claudia Gabriela
- de Paul, Ana Lucia
- Cambiaso, Mariana Lujan
- Torres, Alicia Ines
DOIAbstract
In the present work we aimed at identifying ERα in the plasma membrane of normal anterior pituitary cells and investigated if 17β-estradiol was able to induce their subcellular redistribution. Our results show that about 8% of anterior pituitary cells expressed ERα in the plasma membrane, with the geometrical mean fluorescence intensity being increased after steroid hormone treatment. 17β-Estradiol and the selective ERα agonist PPT induced an increase of ERα expression in the plasma membrane and activated the PKCα/ERK 1/2 pathway in a time-course not compatible with genomic actions, thus supporting the notion of membrane-initiated effects. These findings suggest that 17β-estradiol stimulates the translocation of endogenous ERα to the plasma...
Add to ORKG