Components of the extender and seminal plasma can alter the response of ram spermatozoa to cryopreservation

The cryopreservation of sheep semen represents a challenge to increase pregnancy rates in cervical artificial insemination programs. Decreased sensitivity at low temperatures could counteract certain detrimental effects on sperm. It has been reported that the addition of seminal plasma improves the functionality and fertility in cryopreserved ram spermatozoa. However, this role played by the seminal plasma could be altered by the presence of the components of the extender, among them the egg yolk, cryoprotectant commonly used in the media of dilution and cryopreservation.Fil: Ramírez Vasquez, Rafael Ramón Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; ArgentinaFil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Hozbor, Federico Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentin