Highly reusable invertase biocatalyst: biological fibrils functionalized by photocrosslinking
- Machín, Belén
- Chaves, Analia Silvina
- Ávila, César
- Pera, Licia Maria
- Chehín, Rosana Nieves
- Vera Pingitore, Esteban
DOIAbstract
Here we report a novel strategy for the immobilization of invertase using amyloid-like fibrils as a support. Optimal conditions to get Tyr-Tyr covalent binding between invertase and the support were determined using a photocrosslinking approach. The biological fibrils with invertase activity turn into microstructured catalysts according to electron microscopy outcomes. Thermal and storage stability as well as optimal pH and temperature of the enzyme were conserved. Moreover, the immobilized enzyme recovered by low g-force centrifugation retained 83% of its initial enzymatic activity after 15 reuse cycles. Considering that enzyme cost is the most significant part of the overall fee of enzymatic biomass conversion, the highly efficient recove...
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