Influence of Water on Enzymatic Esterification of Racemic Ketoprofen with Ethanol in a Solvent-Free System
- Toledo, Victoria
- Llerena Suster, Carlos Rafael
- Ruscitti, Claudia Beatriz
- Collins, Sebastián Enrique
- Briand, Laura Estefania
DOIAbstract
The effect of water added in the kinetic resolution of rac-ketoprofen with ethanol catalyzed with Candida antarctica lipase B was investigated under reaction conditions and at a molecular level. The reaction was performed at 45 oC for 24 h with the commercial biocatalyst known as Novozym 435. The addition of water from 0.5 to 10 % v/v to the reaction medium enhances the specific activity (from 2 to 4 micromol.mg-1.h^-1), enantiomeric ratio E (1 towards 5), enantiomeric factor EF (0.1 towards 0.5) and the enantiomeric excess towards dexketoprofen (above 50%). At a molecular level, the rate of H/D isotopic exchange of the amide hydrogen of the lipase B of Candida antarctica demonstrated that the enzyme exposed to dehydrated ethanol possesses ...
Add to ORKG