Thioredoxin-1 Attenuates Ventricular and Mitochondrial Postischemic Dysfunction in the Stunned Myocardium of Transgenic Mice
- Perez, María Virginia
- D'Anunzio, Verónica
- Valdez, Laura Batriz
- Zaobornyj, Tamara
- Bombicino, Silvina Sonia
- Mazo, Tamara
- Longo, Nadia Andrea
- Gironacci, Mariela Mercedes
- Boveris, Alberto Antonio
- Sadoshima, Junichi
- Gelpi, Ricardo Jorge
DOIAbstract
Aim: We evaluated the effect of thioredoxin1 (Trx1) system on postischemic ventricular and mitochondrial dysfunction using transgenic mice overexpressing cardiac Trx1 and a dominant negative (DN-Trx1) mutant (C32S/C35S) of Trx1. Langendorff-perfused hearts were subjected to 15 min of ischemia followed by 30 min of reperfusion (R). We measured left ventricular developed pressure (LVDP, mmHg), left ventricular end diastolic pressure (LVEDP, mmHg), and t63 (relaxation index, msec). Mitochondrial respiration, SERCA2a, phospholamban (PLB), and phospholamban phosphorylation (p-PLB) Thr17 expression (Western blot) were also evaluated. Results: At 30 min of reperfusion, Trx1 improved contractile state (LVDP: Trx1: 57.4 ± 4.9 vs. Wt: 27.1 ± 6.3 and ...
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