Development and Design of a Magnetophoresis System

傳統磁性分離(Magnetic Separation)技術能有效的在水溶液中分離磁性粒子及其他分子結構，此方法不僅快速且簡單，但其缺點是無法精細的分離出磁性粒子和帶有磁性粒子的細胞或其他生物分子結構，本論文中所研製之磁泳系統能有 效地解決其問題。 磁泳系統(Magnetophoresis System)的工作原理，是根據梯度磁場作用於磁性粒子上之牽引力與水溶液中的阻力，當兩股力量達到平衡時，磁性粒子會沿著磁場強度增強的方向進行等速直線運動，因此磁場梯度的設計對於磁泳載台開發是非常關鍵的步驟。為了更進一步的釐清磁性粒子運動速度與磁場梯度的相依性，因此在實驗中選用兩種粒徑大小之磁性粒子(2.8 µm 和 4.5 µm)來探討磁性粒子移動速度與梯度磁場強度的關係，結果顯示，設計出來之磁泳系統能有效的分離不同粒徑大小之磁性粒子，此外磁泳系統於水溶液中也可有效的分離出磁性粒子與帶有細胞之磁性粒子。The traditional magnetic separation technology can effectively separate the magnetic particles and other molecular structures in aqueous solution. This method is very quick and simple, but the disadvantage is it cannot separate the magnetic particle and other biological molecule which is coated with the magnetic particle. A magnetophoresis system developed in this paper can effectively solve this problem. The working principle of a magnetophoresis system is based on an induced magnetic attractive force acting on a magnetic particle subjected to a magnetic gradient field and a drag force acting on a moving magnetic particle in an aqueous solution. When these two forces reach equilibrium, the magnetic particle undergoes a linear motion in the direction of increasing magnetic field strength with a constant speed. Thus, the design of the magnetic gradient field is a crucial step in the development of a magnetophoresis system. To further characterize the designed system, the dependency of the speed of magnetic particles and the magnetic gradient fields is investigated. Two different sizes (2.8 µm and 4.8 µm) of magnetic particles are used in the experiments and the correlation of the moving speeds of these particles and the strength of the gradient field is conducted. It shows that the designed magnetophoresis system can effectively differentiate the magnetic particles with different sizes. Moreover, the magnetophoresis system is used to demonstrate that in an aqueous solution, the isolated magnetic particles and the cell bounded with magnetic particles can be separated.致謝...............................................................i 中文摘要...............................................................ii 英文摘要...............................................................iii 目錄...............................................................iv 圖目錄...............................................................v 表目錄...............................................................vii 第一章 緒論...............................................................1 1.1 前言及研究動機...............................................................1 1.2 文獻回顧............................................................... 3 第二章 基礎理論與 ANSYS 模擬及實驗方法 ................................ 8 2.1 電磁場基礎理論 ...............................................................8 2.2 磁性粒子於梯度磁場的運動原理...............................................................9 2.3 ANSYS 梯度磁場模擬...............................................................10 2.4 製作實際線圈...............................................................12 2.5 梯度磁場的計算方法............................................................... 13 2.6 生物功能性磁性微粒............................................................... 13 2.7 實驗設備............................................................... 14 2.7.1 電源供應器(keithley228) ...............................................................14 2.7.2 顯微鏡(Olympus-CKX41) ................................................................... 15 2.7.3 高速 CCD 數位攝影機(PULNiX TM-6741GE) ................................. 16 2.7.4 高斯計(F.W. BELL gauss/tesla meter model 4048) ............................. 17 2.7.5 微流道製作 ...............................................................18 2.8 實驗步驟與磁珠運動分析............................................................... 18 2.8.1 影像分析方法...............................................................19 第三章 結果與討論...............................................................20 3.1 ANSYS 模擬之結果............................................................... 20 3.2 實際量測磁泳載台之梯度...............................................................22 3.3 ANSYS 模擬結果與實際測磁泳載台梯度比對....................................... 23 3.4 磁性微粒運動速率與磁場梯度之相依性及粒徑大小的相關性 .............25 3.5 磁泳載台於細胞分離實際案例 ................................................. 26 第四章 結論與未來研究方向...............................................................28 4.1 結論 ............................................................... 28 4.2 未來研究方向...............................................................28 參考文獻...............................................................2