Body fat reduction effects of cyclodextrins in rats fed high fat diets

This study investigated body fat-suppressive effects of α-cyclodextrin and β-cyclodextrin in rats fed a high-fat diet. The rats were divided into five groups, namely, the high-fat diet (HF) group, the low dose α-cyclodextrin (α-CD low) group, the high dose α-cyclodextrin (α-CD high) group, the low dose β-cyclodextrin (β-CD low) group and the high dose β-cyclodextrin (β-CD high) group. Rats were fed for 9 weeks from 6 weeks of age without affecting energy intake. Results indicated that rats receiving α-cyclodextrin and β-cyclodextrin could significantly (p < 0.05) reduce mesenteric fat, perirenal fat and epididymal fat accumulations. It was found that α-cyclodextrin and β-cyclodextrin intakes could also decrease total cholesterol, triglyceride, high density lipoprotein cholesterol, and low density lipoprotein cholesterol. In conclusion, the results showed that body fat accumulation could be reduced in rats that have consumed a high dosage of α-cyclodextrin or β-cyclodextrin in their diets.本研究將以高油脂的飲食模式，誘導大鼠產生肥胖，同時給予大 鼠α 環狀糊精和β 環狀糊精，評估環狀糊精對於抑制體脂肪生成之功 能。實驗動物分為5 組，高脂控制組(HF)、低劑量α 環狀糊精組(α-CD low 組)、高劑量α 環狀糊精組(α-CD high 組)、低劑量β 環狀糊精組 (β-CD low 組)與高劑量β 環狀糊精組(簡稱β-CD high 組)。本實驗在 不影響攝食量的情況下，給予6 週齡大鼠共9 週之高脂飲食。研究結 果顯示，與高脂控制組相比，餵食α 環狀糊精與β 環狀糊精不會影響 大鼠攝取熱量，且可顯著(p < 0.05)減少大鼠體內之腸繫膜脂肪率、附 睪脂肪率、腎週脂肪率和總體脂肪率，並具有劑量效應。與高脂控制 組相較之下，β 環狀糊精組有降低老鼠血液中總膽固醇、總三酸甘油 酯、高密度脂蛋白膽固醇和低密度脂蛋白膽固醇的效果。綜合以上， 針對添加α 環狀糊精與β 環狀糊精的減少體脂肪的評估實驗，推測攝 取高劑量α 環狀糊精與β 環狀糊精具有助減少油脂吸收和降低體脂肪 的含量和形成。目次 頁數 中文摘要....................................................................................................i 英文摘要.................................................................................................. ii 目次......................................................................................................... iii 表次......................................................................................................... vi 圖次........................................................................................................ vii 1. 緒論......................................................................................................1 1.1. 肥胖...............................................................................................1 1.1.1. 肥胖的定義.............................................................................1 1.1.2. 肥胖的危害.............................................................................2 1.1.3. 導致肥胖的原因.....................................................................2 1.1.4. 肥胖的盛行率.........................................................................4 1.2. 動物實驗模式...............................................................................4 1.3. 膳食纖維.......................................................................................5 1.3.1. 環狀糊精.................................................................................5 1.3.2. alpha-環狀糊精物理特性及生理活性...................................8 1.3.3. beta-環狀糊精物理特性及生理活性.....................................9 2. 實驗目的............................................................................................10 3. 實驗設計與方法...............................................................................11 3.1. 樣品來源.....................................................................................11 3.2. 動物飼養.....................................................................................11 3.3. 實驗模式.....................................................................................13 3.4. 飼料之調配.................................................................................13 3.5. 樣品劑量之換算和給藥.............................................................13 3.6. 動物犧牲、血液取樣、臟器與體脂肪之採集.........................15 3.7. 攝食量與食物利用率之測定.....................................................15 3.8. 體脂肪率之計算.........................................................................16 3.8.1. 臟器脂肪率...........................................................................16 3.8.2. 屠體粗脂率...........................................................................16 3.9. 肝臟中脂質濃度之測定.............................................................16 3.9.1. 肝臟與血清中之三酸甘油酯測定......................................17 3.9.2. 肝臟與血清中總膽固醇測定..............................................17 3.9.3. 血清中高密度脂蛋白膽固醇測定......................................17 3.9.4. 血清中低密度脂蛋白膽固醇之測定..................................18 3.10. 糞便脂質之萃取與測定方法...................................................18 3.11. 統計分析...................................................................................18 4. 結果與討論........................................................................................19 4.1. 肥胖動物模式建立.....................................................................19 4.2. 飼料與攝食量.............................................................................21 4.3. 體重成長與食物利用率.............................................................21 4.4. 環狀糊精對高脂飲食大鼠體脂肪之影響.................................26 4.5. 環狀糊精對血液脂質之影響.....................................................31 4.6. 環狀糊精對臟器相對重量與健康之影響.................................34 4.7. 環狀糊精對肝臟脂質之影響.....................................................34 4.8. 環狀糊精對糞便脂質之影響.....................................................38 5. 總結....................................................................................................40 6. 參考文獻............................................................................................4