Investigation of bacterial community structure and antibiotic resistance and genetic mobility gene abundance in soils fertilized with swine manure.

Through soil sampling pre- and post-manure application and sampling the manure, the project analyzed the bacterial community present in each sample. DNA was extracted individually from each sample in triplicate for qPCR analysis.Reproducible qPCR results for ermB, sulI, intI, ermC, strB and incWrepA were collected and for each reaction the amount of DNA present was determined based on standard control. All samples were normalized for 16S rRNA. For more information on the project, see published jounal article: Lopatto E, Choi J, Colina A, Ma L, Howe A, et al. (2019) Characterizing the soil microbiome and quantifying antibiotic resistance gene dynamics in agricultural soil following swine CAFO manure application. PLOS ONE 14(8): e0220770. https://doi.org/10.1371/journal.pone.022077