Asynchronous generation of oil droplets using a microfluidic flow focusing system

Here, we show that long-term exposure of PDMS based microfluidic droplet generation systems to water can reverse their characteristics such that they generate oil-in-water droplets instead of water-in-oil droplets. The competition between two oil columns entering via the two side channels leads to asynchronous generation of oil droplets. We identify various modes of droplet generation, and study the size, gap and generation rate of droplets under different combinations of oil and water pressures. Oil droplets can also be generated using syringe pumps, various oil viscosities, and different combinations of immiscible liquids. We also demonstrate the ability to dynamically change the gap between the oil droplets from a few hundred microns to just a few microns in successive cycles using a latex balloon pressure pump. This method requires no special equipment or chemical treatments, and importantly can be reversed by long-term exposure of the PDMS surfaces to the ambient air