Phosphate excess increases susceptibility to pathogen infection in rice

Phosphorus (P) is an essential nutrient for plant growth and productivity. Due to soil fixation, however, phosphorus availability in soil is rarely sufficient to sustain high crop yields. The overuse of fertilizers to circumvent the limited bioavailability of phosphate (Pi) has led to a scenario of excessive soil P in agricultural soils. Whereas adaptive responses to Pi deficiency have been deeply studied, less is known about how plants adapt to Pi excess and how Pi excess might affect disease resistance. We show that high Pi fertilization, and subsequent Pi accumulation, enhances susceptibility to infection by the fungal pathogen Magnaporthe oryzae in rice. This fungus is the causal agent of the blast disease, one of the most damaging diseases of cultivated rice worldwide. Equally, MIR399f overexpression causes an increase in Pi content in rice leaves, which results in enhanced susceptibility to M. oryzae. During pathogen infection, a weaker activation of defence‐related genes occurs in rice plants over‐accumulating Pi in leaves, which is in agreement with the phenotype of blast susceptibility observed in these plants. These data support that Pi, when in excess, compromises defence mechanisms in rice while demonstrating that miR399 functions as a negative regulator of rice immunity. The two signalling pathways, Pi signalling and defence signalling, must operate in a coordinated manner in controlling disease resistance. This information provides a basis to understand the molecular mechanisms involved in immunity in rice plants under high Pi fertilization, an aspect that should be considered in management of the rice blast disease.M.B.P. was funded by a “la Caixa” scholarship for PhD studies in Spanish universities. This research was supported by the Ministerio de Ciencia, Innovación y Universidades (MCIU)‐Agencia Estatal de Investigación (AEI) y el Fondo Europeo de Desarrollo Regional (FEDER) (BIO2015‐67212‐R and RTI2018‐101275‐B‐100) and the Academia Sinica, Taiwan (AS‐SS‐106‐03‐3). We also acknowledge support from the CERCA Programme (“Generalitat de Catalunya”) and the “Severo Ochoa Programme for Centres of Excellence in R&D” 2016‐2019 (SEV‐2015‐0533).Peer reviewe