The Study of extract ethanol of Pirdot (Saurauria Vulcani Korth.) as immunostimulant in Rats (Rattus norvegicus)

BACKGROUND: Ethanolic extract of Pirdot leaves (Saurauria vulcani Korth) (EES) has bioactive compound to decrease glucose and increase insulin levels. AIM: To know of effect of ethanolic extract of Pirdot leaves of immunoglobulin G and lysozyme activity in rats treated with Sheep Red Blood Cell (SRBC). METHODS: Experimental design used in this research was Completely Randomized Design (CRD). There were 24 white rats to be classified into four groups: a control group with water (G0), a group treated with 0.1 ml sheep red blood cell (SRBC) (G1), a group treated with 500 mg/kg ethanol extract pirdot leaves (EES) (G2), and a group treated with 500 mg/KgBW ethanol Extract pirdot leaves (EES) + 0.1 ml SRBC (G3). Immunostimulant activity was evaluated by analyzingthe levels of immunoglobulin G with ELISA method, lysozyme with ABX Micros 60 and histophatology kidney with Hematoxylin-Eosin stainning. RESULTS: The lysozyme activity in rats were treated with only SRBC () is higher and significantly different compared with a group of control (). Necrosis was not found in a group control () and rats treated EES 500 mg/kg ().There were no significant change in histophatology of kidney in rats treated EES 500 mg/kg. Disruption in bowman space with glomerulus was found in G1 (SRBC). Antigen induced disruption in G1 (SRBC) whereas giving extract ethanolic Pirdot leaves concurrently with antigen SRBC can protect kidney from disruption in G3. CONCLUSIONS: A significantly effect of immunoglobulin G, lysozyme activity and histophatology kidney in rats after given by ethanolic extract of pirdot leaves