Hi-C protocol for analysis of plant nuclear chromatin interactions

The nuclear space is a dynamic environment in which DNA molecules interact across time, space and scale. Within the nucleus different yet adjacent chromosomes are co- regulated through shared chromatin modifications or the influence of global enhancer or repressor units. The interactions between adjacent chromosomes can be analysed though Hi-C, a technique that takes a whole genome view on samples obtained by chromosome confirmation capture. This technique has been used in many different plant species and this article provides a consensus protocol that is merged from those used in two expert labs