Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): Comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay.

An immunoassay based on fluorescence polarization detection (FPIA) has been recently adapted for dibekacin. This has been compared with a reference method (high-performance liquid chromatography), and two other methods used in clinical laboratories for monitoring this aminoglycoside, namely substrate-labelled fluorescent immunoassay (SLFIA) and radioimmunoassay (RIA). FPIA was fast and more reliable than SLFIA or RIA, and offered therefore superior performance. However, its nominal cost per assay is high