G&T-seq: parallel sequencing of single-cell genomes and transcriptomes
- Macaulay, Iain C.
- Haerty, Wilfried
- Kumar, Parveen
- Li, Yang I.
- Hu, Tim Xiaoming
- Teng, Mabel J.
- Goolam, Mubeen
- Saurat, Nathalie
- Coupland, Paul
- Shirley, Lesley M.
- Smith, Miriam
- Van Der Aa, Niels
- Banerjee, Ruby
- Ellis, Peter D.
- Quail, Michael A.
- Swerdlow, Harold P.
- Zernicka-Goetz, Magdalena
- Livesey, Frederick J.
- Ponting, Chris P.
- Voet, Thierry
DOIAbstract
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone
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