Efficiency of An Automatic Peak Purity Control Procedure in High-performance Liquid-chromatography Photodiode-array Coupling Based On Evolving Factor-analysis

A modified version of evolving factor analysis, a fully multivariate technique promising to check peak purity in liquid chromatography with photodiode array detection, has been commercially implemented. The capabilities of the algorithm are tested on actual chromatographic data. The results may be regarded as quite remarkable considering that no prior information about the solutes is necessary. When using the algorithm, one has however to be aware that the maximal absorbance must be kept lower than 0.4 AU and that a data rate of 1 Hz is required for optimal peak purity assignment, as a result of the influence of non-constant variance and scattering of the spectral data on the computation technique