Add to ORKGThe invention provides methods and kits for detecting potato viruses in a potato plant.In particular, the invention provides for reverse-transcriptase polymerase chain reaction (RT- PCR)-based methods for simultaneously detecting one or more potato virus, preferably selected from Potato leafroll virus (PLRV), Potato virus X (PVX), Potato virus Y (PVY), Potato virus A (PVA), Potato virus M (PVM), and Potato virus S (PVS), and potato actin mRNA as internal control for the RT-PCR. The invention further provides for kits of parts useful in these methods and comprising one or more primer pairs for amplifying viral RNA of one or more potato virus and a primer pair for amplifying potato actin mRNA by RT- PCR, and optionally comprising probes for d...
Healthy, virus tested potato plants of the cultivars Rua, Desiree and Maris Court were inoculated wi...
Viral diseases are among the most limiting factors in the production of potato in Colombia and the r...
Vegetative propagation of seed potato often allows passaging of viruses to seed tubers, resulting in...
Potatoes are developed vegetatively from tubers, and therefore potato virus transmission is always a...
587-590A multiplex reverse transcription polymerase chain reaction (RT-PCR) protocol was evaluated...
A novel isothermal multiplex AmpliDet RNA system is described for the simultaneous amplification and...
A method for the detection of RNA viral and subviral plant pathogens was developed that combines pat...
Abstract Background Certification of seed potato as free of viruses is essential for stable potato p...
A high throughput, real-time multiplex, single tube RT-PCR assay was developed for simultaneous dete...
NASBA, an isothermal amplification method for nucleic acids, was applied to the detection of RNA of ...
The main result is the establishment of molecular detection methods for potato spindle-tuber viroid ...
A rapid method of detection and evaluation of potato virus X (PVX) infection in potato leaves is des...
The primary step for efficient control of viral diseases is the development of simple, rapid, and se...
The surveys conducted during 2006 and 2007 revealed the infection of the virus in potato fields in T...
Potato is important in Western Australia both for domestic food production and export. Four viruses...
Healthy, virus tested potato plants of the cultivars Rua, Desiree and Maris Court were inoculated wi...
Viral diseases are among the most limiting factors in the production of potato in Colombia and the r...
Vegetative propagation of seed potato often allows passaging of viruses to seed tubers, resulting in...
Potatoes are developed vegetatively from tubers, and therefore potato virus transmission is always a...
587-590A multiplex reverse transcription polymerase chain reaction (RT-PCR) protocol was evaluated...
A novel isothermal multiplex AmpliDet RNA system is described for the simultaneous amplification and...
A method for the detection of RNA viral and subviral plant pathogens was developed that combines pat...
Abstract Background Certification of seed potato as free of viruses is essential for stable potato p...
A high throughput, real-time multiplex, single tube RT-PCR assay was developed for simultaneous dete...
NASBA, an isothermal amplification method for nucleic acids, was applied to the detection of RNA of ...
The main result is the establishment of molecular detection methods for potato spindle-tuber viroid ...
A rapid method of detection and evaluation of potato virus X (PVX) infection in potato leaves is des...
The primary step for efficient control of viral diseases is the development of simple, rapid, and se...
The surveys conducted during 2006 and 2007 revealed the infection of the virus in potato fields in T...
Potato is important in Western Australia both for domestic food production and export. Four viruses...
Healthy, virus tested potato plants of the cultivars Rua, Desiree and Maris Court were inoculated wi...
Viral diseases are among the most limiting factors in the production of potato in Colombia and the r...
Vegetative propagation of seed potato often allows passaging of viruses to seed tubers, resulting in...
