Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins

Xu, Chenjie
Xu, Keming
Gu, Hongwei
Zhong, Xiaofen
Guo, Zhihong
Zheng, Rongkun
Zhang, Xixiang
Xu, Bing

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Publication date

January 2004

DOI

10.1021/ja031776d

ISSN

0002-7863

Journal

issn:0002-7863

Citation count (estimate)

329

Abstract

Using Nα,Nα-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt?NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins. Copyright © 2004 American Chemical Society

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Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins

Abstract

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Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins

Abstract

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