Establishment of HPLC-fingerprint analysis for the quality assessment of Angelica sinensis

OBJECITVE: To establish the HPLC-fingerprint analysis for the quality control of Angelica sinensis. METHODS: The HPLC method was used with Hypersil C₁₈ column (200 mm × 4.6 mm, 5 μm). The mobile phases were consisted of acetonitrile-0.01% phosphoric acid (10:90) and methanol-acetonitrile-water (10:30:60) respectively. The flow rate was 0.8 mL·min^-1. The UV detection was performed at 320 nm. 36 batches of Angelica sinensis were determined. RESULTS: The 36 batches of Angelica sinensis were classified to be qualified and unqualified based on the results of cluster analysis and similarity analysis. CONCLUSION: The method was simple and reliable, and it was capable of effectively controlling the quality of Angelica sinensis