Applications of laser Raman spectroscopy in concentration measurements of multiple analytes in human body fluids

The primary goal of these studies was to demonstrate that NIR Raman spectroscopy is feasible as a rapid and reagentless analytic method for clinical diagnostics. Raman spectra were collected on human serum and urine samples using a 785 nm excitation laser and a single-stage holographic spectrometer. A partial least squares method was used to predict the analyte concentrations of interest. The actual concentrations were determined by a standard clinical chemistry. The prediction accuracy of total protein, albumin, triglyceride and glucose in human sera ranged from 1.5% to 5% which is greatly acceptable for clinical diagnostics. The concentration measurements of acetaminophen, ethanol and codeine in human urine have demonstrated the potential of NIR Raman technology in screening of therapeutic drugs and substances of abuse