Amperometric Determination of Phosphoglucomutase Activity with a Bienzyme Screen-printed Biosensor

Phosphoglucomutase (PGM) is a ubiquitous enzyme that is expressed in all organisms from bacteria to plants to animals and controls a key branch point for carbohydrate metabolism. PGM catalyzes the interconversion of glucose-1-phosphate and glucose-6-phosphate. In this process, the enzyme links various catabolic pathways to yield energy ATP or reducing power NAD(P)H, and several anabolic pathways, such as to lead to the synthesis of polysaccharides [1-4]