Inhibitory effects of saliva as a suspending fluid on attachment of oral bacteria to hydroxyapatite and titanium

Objectives: This study aims to examine the influence of saliva on the attachment of oral bacteria to hydroxyapatite and titanium surfaces in an in vitro setting using saliva as a suspending fluid for the bacterial cells, and to investigate the changes in bacterial surface physicochemical properties (hydrophobicity and charge) induced by saliva. Design: Saliva collected from human donors was used to treat five strains of oral bacteria. The surface hydrophobicity and charge of the treated cells were measured. The effects of saliva as a suspending fluid on attachment of the strains to hydroxyapatite and titanium were investigated. Results: Saliva was found to inhibit the attachment of four streptococcal strains by up to 100-fold. The inhibitory effects were potentially due to changes in cell-surface physicochemical properties induced by saliva. These effects were, however, not observed on Actinomyces naeslundii. Conclusions: The results suggest that saliva may reduce bacterial colonization by oral streptococci and that using saliva as a suspending fluid may be a useful addition for bacterial attachment studies