Facile derivative UV spectroscopy method: simultaneous estimation of tinidazole and fluconazole in combined tablet dosage form

Two derivative spectrophotometric methods have been developed for simultaneous determination of tinidazole and fluconazole in pharmaceutical formulations. The first method depends on utilization of first derivative UV spectrophotometry, with zero-crossing and peak-to-base measurement at 260.57 & 264.23 nm for fluconazole and 263.86 & 318.85 nm for tinidazole. The second method, compensation technique depends on first derivative of the ratio-spectra by measurements of the amplitudes for tinidazole and fluconazole. Calibration graphs were established for tinidazole and fluconazole in the range of 10-100 μg ml and 2-20 μg ml , respectively. All proposed methods have been extensively validated. The results were found to be precise and free from interferences. The described methods can be readily utilized for analysis of pharmaceutical formulations. There was no significant difference between the performances of all of the proposed methods regarding the statistical values