High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey

[EN] In this study, a piezoelectric immunosensor based on High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) technology was developed for detection of sulfathiazole in honey. The biorecognition was based on a competitive immunoassay in the conjugate-coated format, using monoclonal antibodies as specific im-munoreagents. The quantification of sulfathiazole was performed by building the corresponding calibration standard curve in diluted honey (1/140). Due to the competitive nature of the immunoassay, the standard curve showed a sigmoidal pattern with limits of detection (LOD) and quantification (LOQ) of 0.10 ¿g/kg and 2 ¿g/kg honey, respectively. The LOD reached by this immunosensor is 40¿50 times lower than those reported by other techniques for antibiotic detection. Moreover, this method requires minimum honey pre-treatment, making it faster and simpler than other methods. This immunosensor meets the precision and accuracy requirements established by SANCO guidelines, when sulfathiazole concentration in honey is not lower than 10 ¿g/kg. These findings could be the basis for reaching enough reliability for lower concentrations. Therefore, HFF-QCMD immunosensors can be considered a feasible alternative to current techniques for rapid and highly sensitive determination of sulfathiazole in honey with minimum sample preparation.The Spanish Ministry of Economy and Competitiveness, with FEDER-funds, has financially supported this work through the AGL 2013-48646-R project. Furthermore, authors are grateful for financial support from the European Social Fund and the Generalitat Valenciana for the PhD grant (Ayudas para la contratación de personal investigador en formación de carácter predoctoral) to Lourdes Cervera Chiner (ACIF/2016/132). The authors would also like to thank the Universitat Politècnica de València for the support provided for contracting Marisol Juan Borrás in the frame of PAID-10-17 (Ayuda para la contratación de acceso al Sistema Español de Ciencia, Tecnología e Innovación en Estructuras de Investigación).Cervera-Chiner, L.; Jiménez Jiménez, Y.; Montoya, Á.; Juan-Borras, MDS.; Pascual, N.; Arnau Vives, A.; Escriche Roberto, MI. (2020). High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey. Food Control. 115:1-6. https://doi.org/10.1016/j.foodcont.2020.1072961611